Microparticles for Suspension Culture of Mammalian Cells

Carrot Suspension Culture Cells

Growth of 293 cells in suspension culture.

A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.

A precise method for replicating suspension cultures of mammalian cells.

A simple, readily assembled shaker-culture system for the cultivation of mammalian cells is described. No specialized glassware and equipment were used in this system, which consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed to quantitate the effects of several variables, including medium ingredients, serial transfers, and freezing and storage on tw...

Catalase Requirement for Mammalian Cells in Culture

The studies of Puck and his coworkers (1) have made possible the growth of single mammalian cells by a procedure comparable to the plating technique of microbiology. Knowledge of the growth requirements peculiar to single cells (2) or small numbers of cells, however, is essentially lacking. Although the mutually advantageous interaction of cells growing close together was indicated by Sanford e...

Growth factors for mammalian cells in culture.

Essentially all cultured animal cells require for growth1 animal serum or some other biological preparation. An understanding of the nature of the contributions made by these complex mixtures is of great interest for many reasons. Included are the desirability of better defined media for biochemical and other studies, the elucidation of growth requirements not detectable in experiments with who...